LC-MS/MS system sensitivity has moved at an alarming rate over the last 10 years or so. The original perception that due to increased instrument sensitivity sample preparation requirements will decrease has been dispelled. This sensitivity can come at a price, in that levels of contamination not previously detected with less sensitive instruments can now have larger impact on analysis. Combining this with the advent of ultra high-pressure liquid chromatography (UHPLC), the necessity for high-purity components and samples is even more apparent. Given this information the choice of sample preparation technique varies between labs and is generally dependent on several factors: operator familiarity; technique complexity; price per sample; instrument sensitivity; assay sensitivity and limits of quantitation; sample volume; throughput; analyte functionality and target matrix.
This webinar will discuss typical clinical matrices such as whole blood, plasma, serum, urine and oral fluid with specific emphasis on a variety of endogenous components. Matrix complexity will be illustrated and related to a variety of clinical assays, demonstrating potential impact on method robustness. Examples will be presented detailing challenges involved for large panel analysis.
By attending this upcoming presentation you will be provided with:
- an introduction to individual matrix components for a variety of biological sample types and potential issues in LC/MS analysis
- consideration of sample preparation technique when dealing with cross-functional analytes in clinical assays.